Professor
Department of Biological Sciences
Environmental and Molecular Toxicology Program
North Carolina State University
Campus Box 7633
Raleigh, NC27695-7633
Phone: 919-513-1106
Fax: 919-515-7169
e-mail: yoshiaki_tsuji@ncsu.edu
Lab: Rooms #2230, #2232, and #2232A, Office: Room #2203
Ph.D. in Cell Biology, Hiroshima University School of Medicine, Hiroshima, Japan
Postdoctoral Fellow, Stanford University School of Medicine, Palo Alto, California
Research Interests
• Signaling pathways leading to transcriptional activation of antioxidant genes under oxidative and genotoxic stress
• Chromatin remodeling and gene regulation
• Disorders of iron homeostasis and cancer, neurodegeneration, and aging
• mRNA stability and microRNAs
• Mitochondrial oxidative stress
Areas of Research
gene regulation, cell signaling, oxidative stress, iron homeostasis, chromatin remodeling
Teaching (team-taught classes)
TOX201/601: Poisons, People and the Environment (Coordinator, Spring)
TOX701: Fundamentals of Toxicology (Fall)
TOX710: Molecular and Biochemical Toxicology (Coordinator, Spring)
CBS770: Cell Biology (Spring)
TOX801: Seminar in Toxicology
Selected Publications
Y. Tsuji
“Optimization of Biotinylated RNA and DNA Pull-Down Assays for Detection of Binding Proteins: Examples of IRP1, IRP2, HuR, AUF1, and Nrf2”
Int. J. Mol. Sci., 24: 3604 (2023)
Y. Tsuji
“Transmembrane protein western blotting: Impact of sample preparation on detection of SLC11A2 (DMT1) and SCL40A1 (ferroportin)”
PLOS ONE, 15(7): e0235563 (2020)
M. Miyazawa, A. R. Bogdan, Y. Tsuji
“Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)”
Cell Chem. Biol., 26: 85-97 (2019)
M. Miyazawa*, A. R. Bogdan*, K. Hashimoto, Y. Tsuji
“Regulation of Transferrin Receptor-1 mRNA by the Interplay between IRE-binding Proteins and miR-7/miR-141 in the 3′-IRE Stem-loops”
RNA, 24: 468-479 (2018)
K. Hashimoto, Y. Tsuji
“Arsenic-induced Activation of the Homeodomain-Interacting Protein Kinase 2 (HIPK2) to cAMP-Response Element Binding Protein (CREB) Axis”
J. Mol. Biol., 429: 64-78 (2017)
K. Hashimoto, R. Majumdar, Y. Tsuji
“Nuclear Lamins and Progerin are Dispensable for Antioxidant Nrf2 Response to Arsenic and Cadmium”
Cell. Signal, 33: 69-78 (2017)
B. R. Wilson, A. R. Bogdan, M. Miyazawa, K. Hashimoto, Y. Tsuji
“Siderophores in Iron Metabolism: From Mechanism to Therapy Potential”
Trends Mol. Med., 22: 1077-1090 (2016)
A. R. Bogdan, M. Miyazawa, K. Hashimoto, Y. Tsuji
“Regulators of Iron Homeostasis: new players in metabolism, cell death, and disease”
Trends Biochem. Sci., 41: 274-286 (2016)
P.D Ray, B.W Huang, and Y. Tsuji
“Coordinated Regulation of Nrf2 and Histone H3 Serine 10 Phosphorylation in Arsenite-activated Transcription of the Human Heme Oxygenase-1 Gene”
BBA – Gene Regulatory Mechanisms, 1849: 1277-1288 (2015)
M. Miyazawa and Y. Tsuji
“Evidence for a Novel Antioxidant Function and Isoform-specific Regulation of the Human p66Shc Gene”
Mol. Biol. Cell, 25: 2116-2127 (2014)
B.W. Huang, M. Miyazawa, and Y. Tsuji
“Distinct Regulatory Mechanisms of the Human Ferritin Gene by Hypoxia and Hypoxia Mimetic Cobalt Chloride at the Transcriptional and Post-transcriptional Levels”
Cell. Signal., 26: 2702-2709 (2014)
B.W Huang, P.D. Ray, K. Iwasaki, and Y. Tsuji
“Transcriptional Regulation of the Human Ferritin Gene by Coordinated Regulation of Nrf2 and Protein Arginine Methyltransferases PRMT1 and PRMT4”
FASEB J., 27: 3763-3774 (2013)
K. Iwasaki, P.D. Ray, B.W Huang. K. Sakamoto, T. Kobayashi, Y Tsuji
“Role of AMP-activated Protein Kinase in Ferritin H Gene Expression by Resveratrol in Human T Cells”
Biochemistry, 52: 5075-5083 (2013)
P.D Ray, B.W Huang, and Y. Tsuji
“Reactive Oxygen Species (ROS) Homeostasis and Redox Regulation in Cellular Signaling”
Cell. Signal., 24: 981-990 (2012)
K. Hailemariam, K. Iwasaki, B-W. Huang, K. Sakamoto, and Y. Tsuji
“Transcriptional regulation of ferritin and antioxidant genes by HIPK2 under genotoxic stress”
J. Cell Sci., 123: 3863-3871 (2010)
K. Sakamoto, B-W. Huang, K. Iwasaki, K. Hailemariam, J. Ninomiya-Tsuji and Y. Tsuji
“Regulation of Genotoxic Stress Response by Homeodomain-interacting Protein Kinase 2 through Phosphorylation of Cyclic AMP Response Element-binding Protein at Serine 271”
Mol. Biol. Cell, 21: 2966-2974 (2010)
K. Sakamoto, K. Iwasaki, H. Sugiyama and Y. Tsuji
“Role of the Tumor Suppressor PTEN in Antioxidant Responsive Element-mediated Transcription and Associated Histone Modifications”
Mol. Biol. Cell, 20: 1606-1617 (2009)
E.L. MacKenzie, P. D. Ray and Y. Tsuji
“Role and Regulation of Ferritin H in Rotenone-mediated Mitochodrial Oxidative Stress”
Free Radic. Biol. Med., 44: 1762-1771 (2008)
E.L. MacKenzie and Y. Tsuji
“Elevated Intracellular Calcium Increases Ferritin H Expression through an NFAT-independent Posttranscriptional Mechanism Involving mRNA Stabilization”,
Biochem. J., 411: 107-113 (2008)
E.L. MacKenzie, K. Iwasaki and Y. Tsuji
“Intracellular Iron Transport and Storage: From Molecular Mechanisms to Health Implications”
Antioxid. Redox Signaling, 10: 997-1030 (2008)
K. Iwasaki, K. Hailemariam and Y. Tsuji
“Protein Inhibitor of Activated STAT3 (PIAS3) Interacts with Activating Transcription Factor 1 (ATF1) and Regulates an Antioxidant Responsive Element of the Human Ferritin H Gene”
J. Biol. Chem., 282: 22335-22343 (2007)
K. Iwasaki, E. L. MacKenzie, K. Hailemariam, K. Sakamoto and Y. Tsuji
“Hemin-mediated Regulation of an Antioxidant Responsive Element of the Human Ferritin H Gene and Role of Ref-1 during Erythroid Differentiation of K562 Cells”
Mol. Cell. Biol., 26: 2845-2856 (2006)
Y. Tsuji
“JunD Activates Transcription of the Human Ferritin H Gene through an Antioxidant Response Element during Oxidative Stress”
Oncogene, 24: 7567-7578 (2005)